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返回产品中心>蛋白酶体zui通用的形式就是26s蛋白酶体,它包含一个20S的核心结构颗粒和两个19S调节调节基团。所有20S的核心结构颗粒包含四对七聚物环状结构组成两种不同的亚基,alpha亚基是结构亚基,beta亚基主要是催化亚基。
产品描述:
蛋白酶体zui通用的形式就是26s蛋白酶体,它包含一个20S的核心结构颗粒和两个19S调节调节基团。所有20S的核心结构颗粒包含四对七聚物环状结构组成两种不同的亚基,alpha亚基是结构亚基,beta亚基主要是催化亚基。在两个环状结构外每个含有七个alpha亚基主要是为了调整颗粒的停靠。Alpha亚基N端结构是可以阻挡未调节底物进入内腔的门户。内腔两个环分别包含七个beta亚基包含酶激活位点来执行蛋白水解反应。Amplite 20S蛋白酶体荧光检测试剂盒可以用来检测培养细胞或者细胞裂解液里蛋白酶体复合物中类似*蛋白酶的活性。这个试剂盒使用LLVY-R110作为蛋白酶的荧光指示剂。通过蛋白酶切开LLVY-R110产生强绿色的R110,其检测荧光在520-530nm,激发光为480-500nm。该试剂盒提供所有必要成分并提供*实验操作方案。这个实验能稳定并高通量的评估从培养细胞或者溶液中蛋白酶体的活性和筛选抑制剂。该实验方案能方便的在96或384微孔板中分析。
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英文注释:
Our Amplite Fluorometric 20S Proteasome Assay Kit is a homogeneous fluorescent assay that measures the chymotrypsin-like protease activity associated with the proteasome complex either in cultured cells or cell lysates. This kit uses LLVY-R110 as a fluorogenic indicator for proteasome activity. Cleavage of LLVY-R110 by proteasome generates the strongly green fluorescent R110 that is monitored fluorimetrically at 520-530 nm with excitation of 480-500 nm. The kit provides all the essential components with an optimized assay protocol. The assay is robust, and can be readily adapted for high-throughput assays for evaluation of proteasome activity or inhibitor screening in cultured cells or in solution. The assay can be performed in a convenient 96-well and 384-well fluorescence microtiter-plate format. The main function of the proteasome is to degrade unneeded or damaged proteins by proteolysis, a chemical reaction that breaks peptide bonds. The proteasomal degradation pathway is essential for many cellular processes, including the cell cycle, the regulation of gene expression, and responses to oxidative stress. The most common form of the proteasome in this pathway is the 26S proteasome, an ATP-dependent proteolytic complex, which contains one 20S (700-kDa) core particle structure and two 19S (700-kDa) regulatory caps. The 20S core contains three major proteolytic activities including chymotrypsin-like, trypsin-like and caspase-like. It is responsible for the breakdown of key proteins involved with apoptosis, DNA repair, endocytosis, and cell cycle control.
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5(6)-FAM,SE[5(6)-羧基荧光素琥珀酰亚胺脂]117548-22-8*验证肽 | 111 | 100 mg |
5(6)-FAM,SE[5(6)-羧基荧光素琥珀酰亚胺脂]*验证肽和寡核苷酸标 | 112 | 1 g |
5-FAM,SE[5-羧基荧光素琥珀酰亚胺脂] *单体* | 113 | 10 mg |
5-FAM, SE[5-羧基荧光素琥珀酰亚胺脂]*验证多肽标记验证* | 114 | 100 mg |
5-FAM, SE[5-羧基荧光素琥珀酰亚胺脂]*验证多肽标记验证* | 115 | 1 g |
5-FITC [异硫氰酸荧光素] *超纯级* | 120 | 100 mg |
5-FITC [异硫氰酸荧光素] *超纯级* | 121 | 1 g |
5-FITC [异硫氰酸荧光素] *超纯级* | 122 | 10 g |
5(6)-FAM 乙二胺 | 123 | 100 mg |
5-FAM 乙二胺 | 124 | 100 mg |
5(6)-FAM 尸胺 | 127 | 100 mg |
5(6)-FAM 乙二胺 | 128 | 100 mg |
5-FITC 尸胺 | 129 | 100 mg |
AMF,4-胺甲基荧光素CAS:91539-64-9 | 206 | 25 mg |
荧光素-5-马来酰亚胺 | 130 | 25 mg |
5-IAF[5-吲哚乙酰氨基荧光素] | 222 | 25 mg |
Cyanine 3.5 amine [equivalent to Cy3.5® amine] | 139 | 1 mg |
5-FAM 乙二胺 | 124 | 100 mg |
5(6)-FAM 尸胺 | 127 | 100 mg |
5(6)-FAM 乙二胺 | 128 | 100 mg |
AMF[4‘-胺甲基荧光素] | 206 | 25 mg |
Texas Red® hydrazide *Single Isomer* | 481 | 5 mg |
5-(4,6-二氯三嗪基)氨基荧光素 51306-35-5 | 200 | 25 mg |
6-羧基荧光素3301-79-9 *单体* | 106 | 100 mg |
6-羧基荧光素*验证肽和寡核苷酸标记* | 107 | 1 g |
6-羧基荧光素*验证肽和寡核苷酸标记* | 108 | 5 g |
6-FAM,SE[6-羧基荧光素琥珀酰亚胺脂] *单体* | 116 | 10 mg |
6-FAM, SE [6-羧基荧光素琥珀酰亚胺脂]*标验证标记核苷酸* | 117 | 100 mg |
6-FAM, SE [6-羧基荧光素琥珀酰亚胺脂]*标验证标记核苷酸* | 118 | 1 g |
5-FAM-X, SE | 119 | 5 mg |
6-JOE, SE[6-羧基-4’,5’-二氯-2’,7’-二甲氧基荧光素琥珀酰 | 203 | 5 mg |
5(6)-FAM,SE[5(6)-羧基荧光素琥珀酰亚胺脂]*验证肽和寡核苷酸标 | 112 | 1 g |
5-FAM,SE,[5-羧基荧光素琥珀酰亚胺脂] *单体* | 113 | 10 mg |
5-FAM 炔烃化合物 (5-FAM Alkyne) | 132 | 10 mg |
6-FAM叠氮化合物 (6-FAM Azide) | 133 | 10 mg |
6-FAM 炔烃化合物 ( 6-FAM Alkyne) | 134 | 10 mg |
6-HEX 叠氮化合物 6-HEX azide | 240 | 5 mg |
6-TET 炔烃化合物(6-TET alkyne) | 245 | 5 mg |
6-JOE 炔氢化合物(6-JOE alkyne) | 249 | 5 mg |
活体成像试剂萤火虫荧光素2591-17-5 | 12502-1 | 100mg |
Fluorescein *Fluorescence reference standard* | 1 | 1g |
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