产品描述:
Screen Quest检测试剂盒是一类用于检测各种细胞离子通道以及电位等系列工具,包括膜电位、钙离子流、多药物抗性、氯离子以及氯离子通道等方面的指标。每种检测方案均能提供不同的检测方案。这些高效的检测方案为从多角度研究细胞功能活动提供了一种十分有效的方法。肿瘤细胞对细胞毒素药物的抗性被认为是成功进行*的主要障碍之一。一些肿瘤从一开始就对细胞毒素药物无任何反应,另一些肿瘤细胞一开始会被细胞毒素药物抑制,但肿瘤细胞再次生长,并且具有细胞毒素药物抗性。这种现象可能是由给予的抗癌药物诱导的基因突变所造成,或者它们可能是在恶性肿瘤细胞中本身就存在一类代表性抗性细胞。多药物抗性(MDR)是导致多种*方式失败的主要原因。在过去的几年中发现,肿瘤细胞的*抗性与至少两种ATP依赖性药物流出泵基因的过表达相关,这一观点逐渐广泛地被人们所接受。这些细胞膜蛋白,被称为P-糖蛋白(Pgp, MDR1)和多药物抗性关联蛋白(MRP1),它们都是ABC转运蛋白家族成员。
产品性能:
Screen Quest多药物抗性MDR荧光法检测试剂盒用一种MDR荧光指示剂来检测这两种MDR泵的活性。这种疏水性荧光染料分子快速地穿透细胞膜,并且在细胞中被捕获。经过短暂的孵育,细胞中游离的染料浓度会明显地增加。在MDR1和/或表达MRP1的细胞中,这种染料被MDR转运蛋白转运出来,因此细胞荧光强度下降。然而,当其转运位点被一种干扰了MDR1和/或MRP1泵活性的试剂封闭,细胞荧光强度会显著地增强。MDR检测试剂盒提供所有检测必需组分,并带有一种*检测方法。
产品应用领域:
本检测法可以便捷地用于96或者384微孔板分析中,且易于实现自动化。本检测试剂盒是高通量筛选MDR泵抑制剂或者鉴定细胞是否具有高活性MDR泵的理想试剂盒。
产品英文注释:
Tumor cell resistance to cytotoxic drugs is considered one of the major obstacles to successful chemotherapy. Some tumors are initially resistant and never respond to cytostatic drug treatment; others initially respond well but eventually regrow and become resistant. This phenomenon may result from genetic mutations induced by the administered antitumor agent, or may represent the selection of preexisting resistant cell populations in the malignant tumor. Multi-drug resistance (MDR) is a major factor in the failure of many forms of chemotherapy. In the past few years it has become widely accepted that the resistance to chemotherapy correlates with the overexpression of at least two ATP-dependent drug-efflux pumps. These cell membrane proteins, called P-glycoprotein (Pgp, MDR1), and multidrug-resistance-associated protein (MRP1) are members of the ABC transporter family. Our assay kit uses a fluorescent MDR indicator for assaying these two MDR pump activities. This hydrophobic fluorescent dye molecule rapidly penetrates cell membranes and becomes trapped in cells. Following a short incubation, the intracellular free dye concentration can increase significantly. In the MDR1 and/or MRP1-expressing cells this dye is extruded by the MDR transporter, thus decreasing the cellular fluorescence intensity. However, when its extrusion is blocked by an agent that interferes with the MDR1 and/or MRP1 pump-activity, its cellular fluorescence intensity increases significantly. Our MDR assay kit provides all the essential components with an optimized assay method. The assay can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation. This assay kit is ideal for high throughput screening of MDR pump inhibitors or identifying the cells that have high level of MDR pump activities.
其它产品:
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iFluor™ 790酸 | 1360 | 5 mg |
iFluor 790琥珀酰亚胺酯 | 1368 | 1 mg |
iFluor 350蛋白质标记试剂盒 *每次可标记100ug抗体* | 1220 | 2 Labelings |
iFluor 555蛋白质标记试剂盒 *每次可标记100ug抗体* | 1227 | 2 Labelings |
iFluor 594蛋白质标记试剂盒 *每次可标记100ug抗体* | 1230 | 2 Labelings |
iFluor 647蛋白质标记试剂盒 *每次可标记100ug抗体* | 1235 | 2 Labelings |
iFluor 680蛋白质标记试剂盒 *每次可标记100ug抗体* | 1240 | 2 Labelings |
iFluor 700蛋白质标记试剂盒 *每次可标记100ug抗体* | 1245 | 2 Labelings |
iFluor 750蛋白质标记试剂盒 *每次可标记100ug抗体* | 1250 | 2 Labelings |
iFluor 780蛋白质标记试剂盒 *每次可标记100ug抗体* | 1255 | 2 Labelings |
ReadiLink iFluor 633蛋白标记试剂盒 *小剂量优化尤其适合每次标记100u | 1260 | 2 Labelings |
ReadiLink iFluor 790蛋白标记试剂盒 *小剂量优化尤其适合每次标记100u | 1265 | 2 Labelings |
ReadiLink™ FITC抗体标记试剂盒 | 1299 | 2 Labelings |
iFluor 790 胺 | 1362 | 1 mg |
iFluor 790酰肼 | 1364 | 1 mg |
iFluor 790马来酰亚胺 | 1366 | 1 mg |
Stain-All蛋白及RNA/DNA核酸染料 *超纯级* | 10050 | 25 mg |
Amplite 荧光法荧光胺蛋白质定量试剂盒 *蓝色荧光* | 11100 | 1 kit |
加州红 SE | 479 | 1 mg |
荧光胺 超级纯 | 820 | 25 mg |
NBD-F[4-氟-7-硝基苯并呋咱] 超级纯 | 821 | 5 mg |
NBD-Cl[4-氯-7-硝基苯并呋咱] 超级纯 | 825 | 25 mg |
NBD-X 酸 | 828 | 1 g |
NBD-X, 琥珀酰亚胺酯 | 829 | 100 mg |
DSG [Di(N-succinimidyl) glutarate] | 4520 | 25 mg |
SMCC [(N-马来酰亚胺甲基)环己烷-1-羧酸琥珀酰亚胺酯] | 4501 | 25 mg |
SMCC [(N-马来酰亚胺甲基)环己烷-1-羧酸琥珀酰亚胺酯] | 4502 | 1 g |
SMCC Plus *增强了水溶性,交联效率和稳定性* | 4503 | 5 mg |
磺基-SMCC [(N-马来酰亚胺甲基)环己烷-1-羧酸琥珀酰亚胺酯,钠盐] | 4505 | 25 mg |
3-马来酰亚胺基丙酸- N-琥珀酰亚胺脂 | 4507 | 100 mg |
ReadiLink™RPE抗体标记试剂盒*微尺度优化标签50微克每抗体反应* | 1310 | 2 Labelings |
ReadiLink™APC抗体标记试剂盒*微尺度优化标签50微克每抗体反应* | 1311 | 2 Labelings |
ReadiLink™PERCP抗体标记试剂盒*微尺度优化标签50微克每抗体反应* | 1312 | 2 Labelings |
BSA偶联试剂盒 | 5501 | 1 kit |
KLH偶联试剂盒 | 5502 | 1 kit |
蛋白质*化偶联试剂盒 | 5521 | 1 kit |
Amplite 荧光法*定量检测试剂盒 | 5522 | 200 tests |
Amplite 荧光马来酰亚胺定量试剂盒 *绿色荧光* | 5523 | 1 kit |
Amplite 硫醇荧光法定量分析试剂盒 *绿色荧光* | 5524 | 1 kit |
Amplite 比色法马来酰亚胺定量检测试剂盒 | 5525 | 1 kit |
mFluor™ Violet 450 SE | 1150 | 1 mg |
mFluor™ Violet 510 SE | 1151 | 1 mg |
mFluor™ Violet 540 SE | 1152 | 1 mg |
mFluor™ Blue 570 SE | 1160 | 1 mg |
mFluor™ Green 620 SE | 1165 | 1 mg |
mFluor™ Yellow 630 SE | 1170 | 1 mg |
mFluor™ Red 700 SE | 1190 | 1 mg |
mFluor™ Red 780 SE | 1191 | 1 mg |
mFluor Violet 450蛋白质标记试剂盒 *每次反应可标记100ug抗体* | 1100 | 2 Labelings |
mFluor Violet 420蛋白质标记试剂盒 *每次可标记100ug抗体* | 1105 | 2 Labelings |