产品描述:
Screen Quest检测试剂盒是一类用于检测各种细胞离子通道以及电位等系列工具,包括膜电位、钙离子流、多药物抗性、氯离子以及氯离子通道等方面的指标。每种检测方案均能提供不同的检测方案。这些高效的检测方案为从多角度研究细胞功能活动提供了一种十分有效的方法。肿瘤细胞对细胞毒素药物的抗性被认为是成功进行*的主要障碍之一。一些肿瘤从一开始就对细胞毒素药物无任何反应,另一些肿瘤细胞一开始会被细胞毒素药物抑制,但肿瘤细胞再次生长,并且具有细胞毒素药物抗性。这种现象可能是由给予的抗癌药物诱导的基因突变所造成,或者它们可能是在恶性肿瘤细胞中本身就存在一类代表性抗性细胞。多药物抗性(MDR)是导致多种*方式失败的主要原因。在过去的几年中发现,肿瘤细胞的*抗性与至少两种ATP依赖性药物流出泵基因的过表达相关,这一观点逐渐广泛地被人们所接受。这些细胞膜蛋白,被称为P-糖蛋白(Pgp, MDR1)和多药物抗性关联蛋白(MRP1),它们都是ABC转运蛋白家族成员。
产品性能:
Screen Quest多药物抗性MDR荧光法检测试剂盒用一种MDR荧光指示剂来检测这两种MDR泵的活性。这种疏水性荧光染料分子快速地穿透细胞膜,并且在细胞中被捕获。经过短暂的孵育,细胞中游离的染料浓度会明显地增加。在MDR1和/或表达MRP1的细胞中,这种染料被MDR转运蛋白转运出来,因此细胞荧光强度下降。然而,当其转运位点被一种干扰了MDR1和/或MRP1泵活性的试剂封闭,细胞荧光强度会显著地增强。MDR检测试剂盒提供所有检测必需组分,并带有一种*检测方法。
产品应用领域:
本检测法可以便捷地用于96或者384微孔板分析中,且易于实现自动化。本检测试剂盒是高通量筛选MDR泵抑制剂或者鉴定细胞是否具有高活性MDR泵的理想试剂盒。
产品英文注释:
Tumor cell resistance to cytotoxic drugs is considered one of the major obstacles to successful chemotherapy. Some tumors are initially resistant and never respond to cytostatic drug treatment; others initially respond well but eventually regrow and become resistant. This phenomenon may result from genetic mutations induced by the administered antitumor agent, or may represent the selection of preexisting resistant cell populations in the malignant tumor. Multi-drug resistance (MDR) is a major factor in the failure of many forms of chemotherapy. In the past few years it has become widely accepted that the resistance to chemotherapy correlates with the overexpression of at least two ATP-dependent drug-efflux pumps. These cell membrane proteins, called P-glycoprotein (Pgp, MDR1), and multidrug-resistance-associated protein (MRP1) are members of the ABC transporter family. Our assay kit uses a fluorescent MDR indicator for assaying these two MDR pump activities. This hydrophobic fluorescent dye molecule rapidly penetrates cell membranes and becomes trapped in cells. Following a short incubation, the intracellular free dye concentration can increase significantly. In the MDR1 and/or MRP1-expressing cells this dye is extruded by the MDR transporter, thus decreasing the cellular fluorescence intensity. However, when its extrusion is blocked by an agent that interferes with the MDR1 and/or MRP1 pump-activity, its cellular fluorescence intensity increases significantly. Our MDR assay kit provides all the essential components with an optimized assay method. The assay can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation. This assay kit is ideal for high throughput screening of MDR pump inhibitors or identifying the cells that have high level of MDR pump activities.
其它产品:
产品名称 | 货号 | 规格 |
Z-DEVD-ProRed™ 620 | 13433 | 1 mg |
Z-IETD-ProRed™620 | 13434 | 1 mg |
Z-LEHD-ProRed™ 620 | 13435 | 1 mg |
mFluor™ 450-VAD-FMK | 13475 | 25 Tests |
mFluor™ 510-VAD-FMK | 13476 | 25 Tests |
Caspase 3荧光底物 / Ac-DEVD-AFC | 13401 | 5 mg |
Caspase 3荧光底物 Ac-DEVD-AMC | 13402 | 5 mg |
Caspase 3抑制剂 Ac-DEVD-CHO | 13403 | 1 mg |
Caspase 3显色底物 Ac-DEVD-pNA | 13405 | 5 mg |
Caspase-3荧光标记抑制剂 FITC-C6-DEVD-FMK | 13406 | 1 mg |
Caspase-9荧光标记抑制剂 FITC-C6-LEHD-FMK | 13407 | 1 mg |
FITC-C6-DEVD-FMK | 13408 | 100 ug |
FITC-C6-LEHD-FMK | 13409 | 100 ug |
Caspase 8荧光底物Ac-IETD-AFC | 13410 | 5 mg |
Caspase 8荧光底物Ac-IETD-AMC | 13411 | 5 mg |
Caspase 8抑制剂 Ac-IETD-CHO | 13412 | 5 mg |
Caspase 8显色底物Z-IETD-pNA | 13413 | 5 mg |
D-氨基虫荧光素 | 13415 | 10 mg |
Caspase 3荧光底物 Z-DEVD-AFC | 13420 | 5 mg |
Caspase 3荧光底物 Z-DEVD-AMC | 13421 | 5 mg |
Caspase 3显色底物 Z-DEVD-pNA | 13422 | 5 mg |
Caspase 8荧光底物Z-IETD-AFC | 13425 | 5 mg |
Caspase 9荧光底物Ac-LEHD-AMC | 13426 | 5 mg |
Caspase 9荧光底物(Ac-LEHD)2-R110 | 13427 | 1 mg |
Caspase 9荧光底物(Z-DEVD)2-R110 | 13430 | 1 mg |
Caspase 8荧光底物 (Ac-IETD)2-R110 | 13431 | 1 mg |
FAM-VAD-FMK | 13470 | 25 Tests |
TF4-VAD-FMK | 13471 | 25 Tests |
SRB-VAD-FMK [Sulforhodamine B-VAD-FMK] | 13472 | 25 Tests |
iFluor™700山羊抗-兔子免疫球蛋白*交叉吸收* | 16714 | 200 ug |
Annexin V-mFluor Violet 450偶联物 | 20080 | 100 tests |
Annexin V-mFluor Violet 510偶联物 | 20081 | 100 tests |
Annexin V, 重组的 | 20014 | 100 ug |
Annexin V, 重组的 | 20015 | 1 mg |
Annexin V, FITC标记的 | 20030 | 100 tests |
Annexin V, TRITC标记的 | 20031 | 100 tests |
Annexin V-iFluor 350偶联物 | 20070 | 100 tests |
Annexin V-iFluor 488偶联物 | 20071 | 100 tests |
Annexin V-iFluor 555偶联物 | 20072 | 100 tests |
Annexin V-iFluor 594偶联物 | 20073 | 100 tests |
Annexin V-iFluor 647偶联物 | 20074 | 100 tests |
Annexin V-iFluor 680偶联物 | 20075 | 100 tests |
Annexin V-iFluor 750偶联物 | 20076 | 100 tests |
Annexin V-iFluor 700偶联物 | 20077 | 100 tests |
Cell Meter活细胞Caspase 3/7结合分析试剂盒 *绿色荧光* | 20100 | 25 Tests |
Cell Meter活细胞Caspase 3/7结合分析试剂盒 *红色荧光* | 20101 | 25 Tests |
Cell Meter活细胞Caspase 1结合分析试剂盒 *绿色荧光* | 20108 | 25 Tests |
Cell Meter活细胞Caspase 2结合分析试剂盒 *绿色荧光* | 20111 | 25 Tests |
Cell Meter活细胞Caspase 6结合分析试剂盒 *绿色荧光* | 20113 | 25 Tests |
Cell Meter活细胞Caspase 8结合分析试剂盒 *绿色荧光* | 20115 | 25 Tests |