肾素是参与体内肾素-血管紧张素系统的一种酶,它可以介导胞外体积和动脉血管的收缩。它调节血压和电解质平衡。血管紧张素II可诱导血管压缩并导致血压升高。它也能增加ADH和醛固酮的分泌,刺激下丘脑来激活饥渴反应。
产品描述:
肾素是参与体内肾素-血管紧张素系统的一种酶,它可以介导胞外体积和动脉血管的收缩。它调节血压和电解质平衡。血管紧张素II可诱导血管压缩并导致血压升高。它也能增加ADH和醛固酮的分泌,刺激下丘脑来激活饥渴反应。过度活化肾素-血管紧张素系统导致血管收缩和保持钠和水。肾素已经被证实是治疗高血压一个显著指标。
产品优势:
Amplite肾素检测试剂盒提供了一个简便的实验方案,利用我们专有的 iFluor 488/TQ 520荧光共振能量转换肽,高通量的筛选肾素抑制剂或检测肾素的活性。在荧光共振能量转换肽中,iFluor 488荧光性被TQ 520所淬灭。在裂解成两个独立片段后,iFluor 488荧光性被恢复,这种荧光信号很容易被荧光酶标仪(Ex/Em=490/520nm)所测定。这个实验比基于EDANS/DABCYL实验大约灵敏50倍。Amplite肾素检测试剂盒能检测到1ng/100ul的肾素。
英文注释:
Our Amplite Fluorometric 20S Proteasome Assay Kit is a homogeneous fluorescent assay that measures the chymotrypsin-like protease activity associated with the proteasome complex either in cultured cells or cell lysates. This kit uses LLVY-R110 as a fluorogenic indicator for proteasome activity. Cleavage of LLVY-R110 by proteasome generates the strongly green fluorescent R110 that is monitored fluorimetrically at 520-530 nm with excitation of 480-500 nm. The kit provides all the essential components with an optimized assay protocol. The assay is robust, and can be readily adapted for high-throughput assays for evaluation of proteasome activity or inhibitor screening in cultured cells or in solution. The assay can be performed in a convenient 96-well and 384-well fluorescence microtiter-plate format. The main function of the proteasome is to degrade unneeded or damaged proteins by proteolysis, a chemical reaction that breaks peptide bonds. The proteasomal degradation pathway is essential for many cellular processes, including the cell cycle, the regulation of gene expression, and responses to oxidative stress. The most common form of the proteasome in this pathway is the 26S proteasome, an ATP-dependent proteolytic complex, which contains one 20S (700-kDa) core particle structure and two 19S (700-kDa) regulatory caps. The 20S core contains three major proteolytic activities including chymotrypsin-like, trypsin-like and caspase-like. It is responsible for the breakdown of key proteins involved with apoptosis, DNA repair, endocytosis, and cell cycle control.
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鬼笔环肽-iFluor 750偶联物 | 23130 | 300 Tests |
鬼笔环肽-iFluor 700偶联物 | 23129 | 300 Tests |
鬼笔环肽-iFluor 680偶联物 | 23128 | 300 Tests |
鬼笔环肽-iFluor 647偶联物 | 23127 | 300 Tests |
鬼笔环肽-iFluor 633偶联物 | 23125 | 300 Tests |
鬼笔环肽-iFluor 594偶联物 | 23122 | 300 Tests |
鬼笔环肽-iFluor 555偶联物 | 23119 | 300 Tests |
鬼笔环肽-iFluor 532偶联物 | 23117 | 300 Tests |
鬼笔环肽-iFluor 514偶联物 | 23116 | 300 Tests |
鬼笔环肽-iFluor 488偶联物 | 23115 | 300 Tests |
鬼笔环肽-iFluor 405偶联物 | 23111 | 300 Tests |
鬼笔环肽-iFluor 350偶联物 | 23110 | 300 Tests |
鬼笔环肽-California Red偶联物 | 23103 | 300 Tests |
鬼笔环肽-四甲基罗丹明偶联物 | 23102 | 300 Tests |
鬼笔环肽-Fluorescein偶联物 | 23101 | 300 Tests |
鬼笔环肽-AMCA偶联物 | 23100 | 300 Tests |
Hexidium碘化物 CAS:211566-66-4 | 17562 | 5 mg |
LDS 751荧光染料,CAS:181885-68-7 | 17561 | 25 mg |
PUR-1染料 | 17560 | 1 mg |
核红 DCS1 | 17552 | 0.5 ml |
核橙 DCS1 | 17551 | 0.5 ml |
核绿 DCS1 | 17550 | 0.5 ml |
核红 LCS1 | 17542 | 0.5 ml |
核橙 LCS1 | 17541 | 0.5 ml |
核绿 LCS1 | 17540 | 0.5 ml |
核黄 [Hoechst S769121] [74681-68-8] | 17539 | 25 mg |
Hoechst 33342 | 17535 | 5 mL |
Hoechst 33342 超级纯 [23491-52-3] | 17533 | 1 g |
Hoechst 33342 超级纯 [23491-52-3] | 17530 | 100 mg |
Hoechst 33258 *20mM水溶液* [23491-45-4] | 17525 | 5 mL |
Hoechst 33258 *超级纯* [23491-45-4] | 17523 | 1 g |
Hoechst 33258,CAS:23491-45-4 | 17520 | 100 mg |
噻唑橙类染料 *10mM水溶液* [107091-89-4] | 17519 | 10 mL |
噻唑橙类染料 *超级纯* [107091-89-4] | 17518 | 100 mg |
碘化丙啶 *10mM水溶液* [25535-16-4] | 17517 | 1 mL |
碘化丙啶 *超级纯* [25535-16-4] | 17516 | 5 g |
碘化丙啶 25535-16-4 | 17515 | 25 mg |
荧光金(羟芪巴脒) [223769-64-0] | 17514 | 10 mg |
DAPI[4,6-二脒基-2-苯基吲哚,二氢氯化物] [28718-90-3] | 17513 | 25 mg |
DAPI[4,6-二脒基-2-苯基吲哚,二氢氯化物] *超级纯* [28718- | 17511 | 100 mg |
DAPI[4,6-二脒基-2-苯基吲哚,二氢氯化物] *超级纯* [28718-9 | 17510 | 10 mg |
4’,6二脒基-2-苯吲哚盐酸 | 17509 | 25 mg |
10mM DAPI水溶液 [28718-90-3] | 17507 | 2 mL |
7-氨基放线菌素 D [7240-37-1] | 17501 | 1 mg |
放线菌素 D [50-76-0] | 17505 | 5 mg |
10mg/mL 吖啶橙溶液 | 17503 | 10 mL |
细胞膜荧光探针DiD标记溶液 | 22033 | 10 mL |
细胞膜荧光探针DiD标记溶液 | 22033 | 10 mL |
细胞膜荧光探针DiD标记溶液 | 22033 | 10 mL |
细胞膜荧光探针DiD标记溶液 | 22033 | 10 mL |
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